Figure 5
d-Arabinose induces cell cycle arrest via autophagy-dependent p38 MAPK signaling in breast cancer cells. (A) Breast cancer cells (MCF-7 and MDA-MB-231) were conditioned with or without various concentration of d-arabinose (0, 20, 50, and 100 mM), total cell proteins harvested, and expression levels of JNK, ERK1/2, p38, and their phosphorylated forms measured by western blot. (B) MDA-MB-231 and MCF-7 cells were treated with the p38 MAPK inhibitor, SB203580 (10 μM), for 2 h prior to d-arabinose (50 mM, 24 h) treatment, and expression levels of p-p38, Atg5, LC3-II, Cyclin B1, p21, and p27 assessed by western blot. Representative data from three separate experiments are shown. * #P < 0.05, ** ##P < 0.01.
