Figure 6

Fibrosis after injection of folic acid is more exacerbated in Dicer cKO mice than in control mice. (A–E) Immunohistological staining for Sirius red, PDGFR-β, F4/80, and α-SMA and histomorphometric quantification (B–E) showing that these stained areas increased in Dicer cKO mice compared with that in control mice on day 28. Data information: Scale bar = 100 µm (Sirius red), and 50 µm (PDGFR-β, F4/80, and α-SMA). Bar graphs show the mean ± standard error of the mean values of 3–5 mice per group. Statistical analysis is performed using analysis of variance with Tukey’s post-hoc analysis. *p < 0.05, **p < 0.01, and ***p < 0.001, compared with the control at the same time point.