Figure 3
Effect of uniaxial compression on fusion in BeWo cells, induced with forskolin (24 µM) for 2 days. (A) Schematic illustrating the 10% uniaxial compression experiment performed along with a graph (B) showing the actual regimen over 48 h. (C,D) Fluorescent images of fused BeWo cells stained for E-cadherin junctional proteins, and (F,G) Syndecan-1 on static vs. 10% uniaxial compression over 2 days. Scale bar is 100 µm; green: Syndecan-1, red: E-cadherin, blue: nuclei. (E,H) Quantification of fusion efficiency calculated using E-cadherin and Syndecan-1, respectively. (Data presented as mean ± standard deviation; n = 5 independent samples, ns not significant, p = 0.916 and p = 0.788, respectively by independent Student’s t-test).
