Figure 10
Cell apoptotic population of Jurkat cells after co-culturing. The diagrams indicate analysis apoptosis of Jurak cells using Annexin V/7AAD double staining approach after co-culturing with (a) Calu6-miR-138-5p (b) calu6-scramble (c) A549-miR-138-5p (d) A549-scramble. The apoptotic cells include four distinct populations, which can be considered Q1 (Necrosis), Q2 (Late Apoptosis), Q3 (Early Apoptosis), and Q4 (Live Cells) based on the gradual loss of red staining. The percentage of alive jurkat cells was 51.8% when they co-cultured with Calu6 (control group) (a) while their alive population was 85.9% when they co-cultured with miR-138-5p-transduced Calu6 cells (b). Meanwhile, the percentage of alive jurkat cells after co-culturing with A549 (control group) and miR-138-5p-transduced A549 cells were 63.6% (c) and 80.5% (d), respectively.
