Figure 5 | Scientific Reports

Figure 5

From: Dihydroartemisinin-driven selective anti-lung cancer proliferation by binding to EGFR and inhibition of NRAS signaling pathway-induced DNA damage

Figure 5

DHA blocked the NRAS signaling pathway and triggered DNA damage in lung cancer cells. A549 and LLC were treated with DHA (10 or 60 μg/ml) for 24 h. DMSO treatment served as control. (A, D) The expression of NRAS, p-ERK1/2 in A549 and LLC was detected by WB. (B, E) The indicators of DNA damage (p53 and γ-H2A.X) in A549 and LLC were assayed through WB. (C, F) Comet assay was applied to analyze the DNA double-strand breakage in A549 and LLC. (G, H) The biomarkers of the cell cycle (Cdc25A, CDK4, CyclinD1) in A549 and LLC were analyzed with WB. (I–J) The expression of NRAS, p-ERK1/2, Cdc25A, CDK4, CyclinD1, p53, and γ-H2A.X in tumor grafts was detected by IHC.

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