Figure 1 | Scientific Reports

Figure 1

From: Real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of cassava brown streak viruses

Figure 1

Amplifications for designed RPA primers for detection of cassava brown streak virus (CBSV) and Uganda cassava brown streak virus (UCBSV). G1 and G2 represent amplifications using RPA primers targeting the coat protein (CP) and cylindrical inclusion (CI) genes, respectively, for CBSV. G3 and G4 represent amplifications using primers targeting the CP and CI genes, respectively, for UCBSV. Lanes A, B and C: CBSV infected plant samples; lane D: Dual (CBSV and UCBSV) infected plant sample; Lanes E and F: UCBSV infected plant samples; lane G: Health control plant; lane NT: Non-template control; lane + ve: Dual (CBSV and UCBSV) infected positive plant sample; and L: 100 bp ladder (New England Biolabs).

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