Figure 1 | Scientific Reports

Figure 1

From: Upregulation of the AMPK-FOXO1-PDK4 pathway is a primary mechanism of pyruvate dehydrogenase activity reduction in tafazzin-deficient cells

Figure 1

PDK4 is upregulated in tafazzin-deficient cells. PDK4 and other isoforms levels were evaluated using qPCR (A) (B) or Western blot (WB) analysis (C) of whole-cell lysates extracted from WT and TAZ-KO myoblasts, differentiation-induced myotubes, and mouse muscle tissue. The figures in panel (C) are representative images with each lane representing one biological replicate. The mRNA expression levels were normalized to the internal control Actb, and protein levels were normalized to NDUFB6. Densitometry was quantified using Image J and statistical significance was determined with an unpaired, two-tailed Student's t-test. The data points represent mean ± S.D. (error bars) for each individual biological replicate of each group. * 0.05 < p < 0.1, ** 0.01 < p < 0.05, *** 0.001 < p < 0.01.

Back to article page