Figure 3
Tregs dampen the cGAS-STING pathway in MM cells. To investigate the impact of Tregs on the antigen presenting molecules in MM cells, human MM cell lines (MM.1S and SK-MM-1) were co-cultured with isolated Tregs from the MM patients at a ratio of 1:0.5 for 24 h. (A) qRT-PCR analysis of the mRNA levels of cGAS, STING, TBK1 and IRF3 in human MM cell lines (MM.1S and SK-MM-1) with or without Tregs co-culture. (B) Western blot analysis of the protein levels of cGAS, STING, TBK1, p-TBK1, IRF3 and p-IRF3 in the MM cells with or without Tregs co-culture. (n = 3 independent experiments; n = 3 technical replicates for qRT-PCR measurement in each experimental sample. Student’s t test). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
