Figure 4
STING agonist counteracts the effect of Tregs on MM cells. STING agonist-4 was applied at 1 μM in the MM cells in the presence of Tregs co-culture (MM cells were co-cultured with isolated Tregs at a ratio of 1:0.5 for 24 h). (A) qRT-PCR analysis of cGAS, STING, TBK1 and IRF3 at the mRNA levels. (B). Western blot analysis of the protein levels of cGAS, STING, TBK1, p-TBK1, IRF3 and p-IRF3. (C). qRT-PCR analsyis of HLA-A, HLA-B, HLA-C, MICA, MICB, and PDL1. (D). ELISA analysis of IL-10, TGF-β1, IL-6, TNF-α and CXCL10 in cell culture supernatant. (n = 3 independent experiments; n = 3 technical replicates for ELISA and qRT-PCR measurement in each experimental sample. One-way ANOVA). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
