Figure 1

Frequency and expression patterns of differentially expressed genes (DEGs). (A) Frequencies of genes that are differentially expressed in the weak (136, DR1-136) and strong (107, DR1-107) GmDR1-overexpressers as compared to the nontransgenic Williams 82 (W82) line 12 h following chitin or phosphate buffer (buffer) treatment. The 143 and 289 DEGs, common to both GmDR1-overexpressers 12 h following buffer and chitin treatments and with at least 1.5-fold change and p \(\le\) 0.05 between W82 and each of the GmDR1-overexpressers, are shown with orange and red circles, respectively. Expression of seven genes (shown with green circle; Table S3) was significantly altered in GmDR1-overexpressers 12 h following chitin and buffer treatments. (B) Frequencies of genes that are differentially expressed in the weak (136, DR1-136) and strong (107, DR1-107) GmDR1-overexpressors as compared to the nontransgenic W82 line 24 h following chitin or phosphate buffer treatment. (C) Heat map displaying the differential expression patterns of the 192 (129 induced and 69 repressed; Dataset S2) chitin-responsive DEGs that were differentially expressed at least by twofold with p 0.001 in each of the GmDR1-overexpressors as compared to W82 12 following chitin treatment. Differential expression levels of these genes 24 h following chitin treatment, and 12 and 24 h following buffer treatment are also presented. (D) Heat map displaying the differential expression patterns of the buffer-responsive 115 DEGs (two induced and 113 repressed; Dataset S3) that were differentially expressed at least by 2- fold and p 0.001 in each of the GmDR1-overexpressors as compared to W82 12 following buffer treatment. Differential expression levels of these genes 24 h following buffer treatment, and 12 and 24 h following chitin treatment are also presented.