Figure 3

A subset of chitin-responsive genes encoding LRR-receptor kinases and NLR proteins are induced in GmDR1-overexpressors following F. virguiforme and H. glycines infection. (A) Significantly enhanced expression levels of LRR-receptor kinases and NLR genes 12 h following chitin treatment (RNA-seq data). Fold changes were calculated by comparing FPKM values of a gene in GmDR1-overexpressors, either DR1-136 (blue bars) or DR-107 (orange bars), with that in the nontransgenic control Williams 82 line 12 h following chitin treatment. Transcription fold changes of the genes were at least twofold and p ≤ 0.05. (B) Significantly enhanced expression levels of receptor kinases and NLR genes 14 days following F. virguliforme infection. Fold changes of the genes between GmDR1-overexpressors and Williams 82 following F. virguliforme infection were determined by RT-qPCR. Data in B and C are mean fold change ± SE determined for either DR1-136 (blue bars) or DR-107 (orange bars), from three biological replications. The transcript levels of soybean Elongation factor 1-b (ELF1-b) gene (Glyma.02g44460) was used as an internal control in RT-qPCR. *, p ≤ 05 and **, p ≤ 01. (C) Significantly enhanced expression levels of receptor kinases and NLR genes 15 days following H. glycines infection. Fold changes of the genes between GmDR1-overexpressors, either DR1-136 (blue bars) or DR-107 (orange bars), and Williams 82 following H. glycines infection were determined by RT-qPCR. RT-qPCR data of all 28 DEGs are presented on Table S7.