Figure 2

Transcriptomics analysis of UHRF1 KO G1 (pre)adipocytes reveals DEGs and significantly altered pathways, including inhibition of adipocyte differentiation. (A) Schematic representation of transcriptomics analysis of whole RNA extracted from the following samples: UHRF1 KO G1 versus NT at preadipocyte stage (day 0) and UHRF1 KO versus NT at adipocyte stage (day 10) of 3T3-L1 cells. (B) The number of statistically significant DEGs in both datasets at the indicated cut-off P-value < 0.05 and FDR < 0.05. Upregulated and downregulated genes are highlighted in red and green, respectively. (C–H) Expression log ratio of top upregulated genes in both data sets of UHRF1 KO cells and its qPCR validation. (I, J) IPA prediction of the top 20 affected canonical pathways in both datasets, and those highlighted in red and green are the percentage of upregulated and downregulated genes in each pathway, respectively. (K) Summary graph of adipocyte dataset that shows the interaction network between the most significant predicted pathways and molecules. The orange line indicates activated entities with a positive z-score, and the blue line indicates inhibited entities with a negative z-score, respectively, and estimated edges are shown as dotted lines. Data are mean ± SEM of 3 independent experiments. Statistical analyses were performed using ordinary one-way ANOVA, *P < 0.05.