Figure 2 | Scientific Reports

Figure 2

From: Metabolic remodeling and calcium handling abnormality in induced pluripotent stem cell-derived cardiomyocytes in dilated phase of hypertrophic cardiomyopathy with MYBPC3 frameshift mutation

Figure 2

Pluripotency of the D-HCM iPSCs and cardiac differentiation of iPSCs into iPSC-CMs. (A). D-HCM iPSCs form several colonies (scale,100 µm). (B). DNA sequencing analysis of D-HCM iPSCs reveals MYBPC3 mutation c.1377delC (red arrow). (C). Immunofluorescence with three pluripotency markers (Nanog, Oct3/4, and Sox2) (scale:50 µm). (D). Flow cytometry of two pluripotency markers (SSEA-4 and TRA-1–60). (E). Immunofluorescence with three germ layer markers: ectoderm (TUJ1), mesoderm (αSMA), endoderm (AFP) (Scale:100 µm). (F). Cardiac induction was performed using the GiWi protocol (Day0-14), purification with a lactate medium (Day15-20), and maturation with a fatty acid medium (Day22-30). In the lower part, photographs show each stage of cardiac differentiation on Day 0, Day 5, Day 10, and Day 50 (Scale:200 µm).

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