Figure 3 | Scientific Reports

Figure 3

From: Metabolic remodeling and calcium handling abnormality in induced pluripotent stem cell-derived cardiomyocytes in dilated phase of hypertrophic cardiomyopathy with MYBPC3 frameshift mutation

Figure 3

Cardiac characteristics of iPSC-CMs. (A). Immunofluorescence for Cardiac Troponin T (cTnT) (Scale: 50 Âµm). (B). Flow cytometry with cTnT (blue wave: negative control, red wave: cTnT positive). (C). Immunofluorescence for MLC2-A and MLC2-V (Scale: 50 Âµm). blue: DAPI, green: MLC2-A, red: MLC2-V. (D). Fluorescence-positive cell count (%) for MLC2-A and MLC2-V in Fig. 2C. iPSC-CMs were classified into three groups according to the expression levels, V > A, V≒A, V < A (V: MLC2-V, A: MLC2-A). (E). Protein expression level of cMYBP-C (Ctrl: N = 4 from 2 cell lines, D-HCM: N = 5 from 1 cell line). (F). mRNA expression level of MYBPC3 (Ctrl: N = 6 from 2 cell lines, D-HCM: N = 6 from 1 cell line).

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