Figure 4 | Scientific Reports

Figure 4

From: Metabolic remodeling and calcium handling abnormality in induced pluripotent stem cell-derived cardiomyocytes in dilated phase of hypertrophic cardiomyopathy with MYBPC3 frameshift mutation

Figure 4

Electron microscopy images of cardiac sarcomeres and mitochondria in iPSC-CMs. (A). Cardiac sarcomere structures. Z-band disruption and sarcomere disarray were observed in D-HCM iPSC-CMs (white arrow) (Scale: 5 µm (upper), 2 µm (middle), 1 µm (lower)). (B). Sarcomere lengths (µm) (Ctrl: N = 71, D-HCM: N = 72). (C). Z-band disruption rate (%) measured by sarcomere units (Ctrl: N = 140, D-HCM: N = 220). “N” represents the sarcomere units in A, B, and C. (D). Mitochondrial structures. More damaged mitochondria were observed in D-HCM iPSC-CMs (white arrow) (Scale: 500 nm). (E). The cristae loss area (%) indicates damaged mitochondria (Ctrl: N = 72, D-HCM: N = 68). (F). Mitochondrial size (um2) (Ctrl: N = 53, D-HCM: N = 42). “N” represents the numbers of mitochondria in D, E, and F. All results of TEM were obtained from Ctrl 1 cell line and D-HCM 1 cell line. n.s. indicates not significant vs. Ctrl; **p < 0.01 vs. Ctrl; ***p < 0.001 vs. Ctrl.

Back to article page