Figure 6
From: A simple protocol to establish a conditionally immortalized mouse podocyte cell line
Induction of podocyte injury model using ADR in vitro. (A) CCK-8 viability analysis showed a reduction in cell viability after treatment with ADR. (B) Changes in WT1 and synaptopodin expression in the NC and ADR groups. (C,D) Intensity analyses of WT1 and synaptopodin in (B) using ImageJ. The apoptosis of MPCs was analyzed using flow cytometry (E) NC and (F) ADR. (G) The percentage of apoptotic MPCs was determined as the average across three independent experiments. The data are presented as the means ± SEM (n = 3–6), **P < 0.01, ***P < 0.001.
