Figure 7
From: A simple protocol to establish a conditionally immortalized mouse podocyte cell line
Induction of the podocyte injury model using LPS in vitro. (A) Viability analysis using the CCK-8 assay showed a reduction in MPC viability after treatment with LPS. (n = 6) (B,C) RT‒qPCR analysis of Il6 and Tlr1 expression in the NC and LPS groups. Apoptosis of MPCs was analyzed using flow cytometry following treatment with (D) NC or (E) LPS. (F) The percentage of apoptotic MPCs was determined as the average across three independent experiments. The data are presented as the means ± SEM (n = 3–6), *P < 0.05, **P < 0.01, ***P < 0.001.
