Table 1 Primers used for RT-qPCR.
From: A simple protocol to establish a conditionally immortalized mouse podocyte cell line
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
Wt1 | AGTGAAATGGACAGAAGGGCAGA | TCCAGATACACGCCGCACAT |
Synpo | GACCAGCCAGATAGAGCAAAGC | GGGGAGACCTAACCCGAGAA |
Thsd7a | ACCTGGGTTTATGGTGTCG | GGTTTGAGGCGGTTGTT |
Nphs1 | TGGCGATTCCTGCCTCCGTT | TTCTGCTGGGAGCCCTCGTT |
Nphs2 | GAGGATGGCGGCTGAGATTC | GGTAGTTGATGCTCCCTTGTGCT |
Slc5a2 | ATGGAGCAACACGTAGAGGC | ATGACCAGCAGGAAATAGGCA |
Scl34a1 | AGTGAATGATGTCCTACAGCGAGA | ACTGGAGATGGCATAGGTGGAT |
Fxyd2 | GACTATGAAACCGTCCGCAAAG | CCCCACAGCGGAACCTTTT |
Cldn1 | GGGGACAACATCGTGACCG | AGGAGTCGAAGACTTTGCACT |
Pax8 | AGAGTCACCCCAGTCGGATTC | GCTGGCTGAAGGTGTCCGTA |
Krt8 | CAAGGTGGAACTAGAGTCCCG | CTCGTACTGGGCACGAACTTC |
Pecam1 | CTGCCAGTCCGAAAATGGAAC | CTTCATCCACCGGGGCTATC |
Flt1 | CCACCTCTCTATCCGCTGG | ACCAATGTGCTAACCGTCTTATT |
Pdgfrb | TTCCAGGAGTGATACCAGCTT | AGGGGGCGTGATGACTAGG |
Gata3 | GAACTGCGGGGCAACCTCTA | GCCTTCGCTTGGGCTTGATA |
SV40 tsA58 | CTTGAAAGGAGTGCCTGGGG | TCCCATTCATCAGTTCCATAGGT |
Tlr1 | TGAGGGTCCTGATAATGTCCTAC | AGAGGTCCAAATGCTTGAGGC |
Il6 | CTGCAAGAGACTTCCATCCAG | AGTGGTATAGACAGGTCTGTTGG |
Gapdh | GCACAGTCAAGGCCGAGAAT | GCCTTCTCCATGGTGGTGAA |
