Table 2 List of antibodies used in the study.
From: A simple protocol to establish a conditionally immortalized mouse podocyte cell line
Antibody | Host | Application (dilution) | Supplier |
|---|---|---|---|
Synaptopodin | Rabbit | WB (1:1000), IF (1:500) | Proteintech, 21064-1-AP |
Synaptopodin | Rabbit | IF (1:200) | Abcam, ab259976 |
Synaptopodin | Mouse | IF (1:100) | Santa Cruz, sc-515842 |
Wilms tumor protein | Rabbit | WB (1:1000), IF (1:100) | Abcam, ab89901 |
Nephrin | Rabbit | IF (1:200) | Abcam, ab216341 |
PECAM1 | Rabbit | WB (1:2000) | Abcam, ab222783 |
KRT8 | Rabbit | WB (1:10,000) | Abcam, ab53280 |
GATA3 | Rabbit | WB (1:2000) | Abcam, ab199428 |
SV40 TAg | Rabbit | WB (1:2000) | Abcam, ab234426 |
GAPDH | Rabbit | WB (1:10,000) | Abcam, ab8245 |
Goat anti-rabbit IgG H&L | NA | WB (1:10,000) | Abcam, ab6721 |
Goat anti-rabbit AF555 | NA | IF (1:500) | Abcam, ab150186 |
Goat anti-mouse AF555 | NA | IF (1:500) | Abcam, ab150114 |
