Figure 4

Metabolic stress. (A) Maximum projections of the nuclear (DAPI, cyan) and cytoskeletal (phalloidin, magenta) structures of ASC52telos transduced with either the AcGFP1 or MAHS transgene following 72 h of media depletion. Scale bars are 300 µm. (B) Quantification of cell population in AcGFP1- and MAHS-expressing ASCs following a 72 h media depletion (ANOVAN, Tukey’s HSD, n = 3). Cell survival quantification was normalized to cells cultured in 100% media for 72 h. Denoted p-values indicate pairwise difference in cell population between the genotypes at each specific media concentration.