Figure 4

Comparing protein abundance associated with immunosuppression in PDS. (a) t-SNE plot of PDS cases comparing the immunological status using T-cell infiltration and PD-L1 expression. “Cold” tumors showing neither CD4/CD8 T-cell tumor infiltration nor PD-L1 expression; “hot” tumors can be divided into “immunogenic” tumors showing either PD-L1 expression or infiltration by CD4/CD8 T-cells or “inflamed” tumors showing both PD-L1 expression and infiltration by CD4/CD8 T-cells¹ (b) Heatmap of markers separating inflammatory “hot” and “cold” PDS cases using Human Genome Organization gene symbols. (c) Box plot comparing dedicated markers between inflammatory “hot” (n = 17) and “cold” (n = 6) PDS cases (n = 24; one case not available/NA) compared using a Wilcoxon Rank Sum Test. (d) Scatter plot of an Enrichr¹¹ analysis using the “GO Biological Process 2021” database of the top 50 proteins correlating to tumor purity. The color code represents significant findings (FDR < 0.05, red) (n = 24). The adjusted p-value, the odds ratio and the log2 of the combined score (dot size) are quantitative and qualitative results of the Enrichr overrepresentation analysis, which briefly reflect the relevance of a given term in association to the proteins (HUGO symbols were used as input).