Figure 3
TUG1 is involved in the regulation of glucose metabolism in microglial activation. (a–g) The expression of IL-1β,TUG1, HK2, LDHA, PFKFB3, GAPDH, and PKM2 mRNA after induced with LPS (100 ng/ml)/IFN-γ(10 ng/ml) and with or without 2-DG for 24 h (n = 3). (h) Time curve of relative expression levels of IL-1β, TUG1, HK2, and LDHA mRNA. (i) Western blot was conducted to determine the expression of the key enzymes HK2, G6PD, and PDH after induced with LPS (100 ng/ml)/IFN-γ(10 ng/ml) and with or without 2-DG for 24 h. The immunoblot image represents three independent experiments with similar results. (j) Statistical analysis of data for the expression of HK2 (n = 3). (k) Statistical analysis of data for the expression of G6PD (n = 3). (l) Statistical analysis of data for the expression of PDH (n = 3). (m–p) The expression of IL-4, Arg1, CD206, and Ym1 mRNA after induced with LPS (100 ng/ml)/IFN-γ (10 ng/ml) and with or without 2-DG for 24 h (n = 3). Error bars indicate SEM. *P < 0.05, **P < 0.01.
