Figure 4
TUG1 knockout altered the expression levels of key enzymes in the glycolytic pathway in microglia. (a–d) The expression of HK2, PFKFB3, GAPDH, and LDHA mRNA after induced with LPS (100 ng/ml)/IFN-γ(10 ng/ml) for 24 h (n = 3). (e) Western blot was conducted to determine the expression of the key enzymes HK2, G6PD, and PDH after induced with LPS (100 ng/ml)/IFN-γ(10 ng/ml) for 24 h. The immunoblot image represents three independent experiments with similar results. (f) Statistical analysis of data for the expression of HK2 (n = 3). (g) Statistical analysis of data for the expression of G6PD (n = 3). (h) Statistical analysis of data for the expression of PDH (n = 3). (i–j) The expression of IL-4, Arg1, CD206, and Ym1 mRNA after induced with LPS (100 ng/ml)/IFN-γ(10 ng/ml) for 24 h (n = 3). (m–p). The ratio of IL-4, Arg1, CD206, and Ym1 expression levels in the TUG1KO group compared to the control group, and the TUG1KO induced with LPS/IFN-γ group compared to the LPS/IFN-γ group (n = 3). Error bars indicate SEM. *P < 0.05, **P < 0.01.
