Figure 1

Expression of V-type H⁺ ATPase and P-type Na⁺/K⁺ ATPase protein in rectum of A. taeniorhynchus larvae. Whole mount of the two-part rectum from larval A. taeniorhynchus acclimated to 1% (A–C) and 24 h post-transfer from 1 to 100% seawater (D–F) showing immunolocalization of V-type H⁺ ATPase protein (Cy5 blue A, C, D, F) to both the anterior and posterior rectum. P-type Na⁺/K⁺ ATPase protein (Cy3 red A, B, D, E) was immunolocalized to the anterior rectum. Figures A and D contain overlays of Cy3 and Cy5 signals. (B and E) contain Cy3 signal only and Figures C and F contain Cy 5 only. Scale bars, 100·μm.