Figure 8

Inhibition of Na⁺ transport at the posterior rectum by V-type H⁺-ATPase, NHE3 and NKCC inhibitors. Na⁺ transport rate was measured at the posterior rectum of A. taeniorhynchus larvae that were reared in seawater before and after application of bafilomycin A1 (V-type H⁺-ATPase inhibitor, 8 μmol l⁻¹), S3226 (NHE3 inhibitor, 1 μmol l⁻¹), bumetanide (NKCC inhibitor, 10 µM) or dimethylsulfoxide (DMSO, inhibitor solvent, 0.8%). Values are expressed as mean ± SEM with n = 9 for bafilomycin, n = 11 for S3226, n = 9 for bumetanide and n = 7 for DMSO. Effects of each treatment were assessed by comparing Na⁺ transport rates before (Control) and after inhibitor or solvent with a paired t-test (p = 0.024 for bafilomycin; p = 0.025 for S3226; p = 0.017 for bumetanide; p = 0.754 for DMSO). Na⁺ transport rates of before inhibitor/solvent did not statistically differ from one another (ANOVA, p = 0.502).