Figure 3

Transcriptome analysis of the NC and HC groups. (A) Scatter plot of RNA-seq data. (B) Heatmap of the differentially expressed genes in transcription level. (C) GO (Gene Ontology) enrichment circle diagram of differentially expressed genes in transcription level (from the outside to the inside, the first circle represents 20 significantly enriched pathways, and the number outside the circle is the coordinate ruler of the number of genes. The second circle represents the number and P-value of background genes in this pathway, and the more genes, the longer the bar. The third circle represents the number of DEGs in this pathway (the red color represents upregulated genes and the blue represents downregulated genes). The fourth circle represents the value of Rich Factor in each pathway). (D) Bubble chart of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of differentially expressed genes in transcription level. (E) Histogram of transcript levels of differentially expressed genes in the thermogenic pathway. X-axis represents 26 selected genes, and Y-axis represents the expression levels of genes from RNA-seq.