Figure 7
Screening and functional verification of the IMF deposition-related candidate gene. (A) Overlap of differentially expressed genes in translation efficiency (TE), oxidative phosphorylation pathway, and translation of nine quadrants. (B) Scatterplot showing the translation efficiency (TE) level of COX10 and mtND4L changes at HC compared with NC. (C) Scatterplot showing the translational level of COX10 and mtND4L changes at HC compared with NC. (D,E) IGV snapshot for RNA-seq and Ribo-seq signals of COX10 (D) and mtND4L (E). (F,G) Quantification of RNA-seq data for COX10 (F) and mtND4L (G). (H,I) Quantification of Ribo-seq data for COX10 (H) and mtND4L (I). (J,K) The mRNA level of COX10 (J) and mtND4L (K). (L) The protein levels of COX10 and mtND4L. Gel images were cropped, and the original gels are presented in Supplementary Fig. S6. (M) Quantitative results of COX10 and mtND4L protein levels. (N–O) The mRNA levels of COX10 (N, n = 6) and mtND4L (O, n = 6) in C2C12 cells after transfected with siNC, siCOX10, and simtND4L. (P) Triglyceride content in C2C12 cells after transfected with siNC, siCOX10, and simtND4L (n = 6). The data are expressed as the mean ± SD. *P-value < 0.05, **P-value < 0.01.
