Figure 3
Anti-mycobacterial activity of shortlisted RibH inhibitors. The figure depicts the representative pictograms obtained on screening of anti-mycobacterial activity of three of the shortlisted compounds namely, A10, A39 and A52 against M. tb H37Rv by microplate Alamar blue assay (MABA). The assay was performed at least three times with three technical replicates in each case. Rifampicin and shortlisted compounds were tested at various concentrations between 50 and 0.09 µg/ml (two-fold serial dilution). In our assay rifampicin exhibited an MIC of 0.09–0.048 µg/ml. (A) Compound A10 exhibited an MIC of 1.56 µg/ml, (B) compound A39 exhibited an MIC of 3.125–1.56 µg/ml, (C) compound A52 exhibited an MIC of 0.78 µg/ml. (D) Tabulation of MIC values obtained for various compounds with respect to rifampicin. The experiments were performed at three times with three technical replicates.
