Figure 1
An overview of experimental design. (a) Map of central and northern Europe showing distribution of I. elegans (green area) and replicated high- (Sweden) and central (Poland) latitude sampling sites26. (b) A full-factorial experimental design where non- and post-wintering cohorts from high- and central latitudes were differentiated based on their growth rate and reared at 24 °C and photoperiod L:D 20:4 h, with the post-winter cohort experiencing wintering conditions, 6 °C and L:D 20:4 h. (c) Diagram showing larval development time and the entrance into pre-final instar (F-1; depicted by the white boxes) for the non- and post-wintering cohorts plus simulated wintering time period (grey box). Note that individuals entered wintering conditions at different dates as explained in the "Methods" section.
