Figure 6
From: Enzymatization of mouse monoclonal antibodies to the corresponding catalytic antibodies
In vitro assays. Reaction conditions included Madin–Darby canine kidney (MDCK) cells (6.0 × 104 cells/cm2), influenza A virus (H1N1; 2,000 PFU/mL), and incubation (48 h at 25 °C). (A) InfA-3L/wt and InfA-3L/P95(−). InfA-3L/wt (12 μM; n = 2) exhibited no neutralization effect; however, InfA-3L/P95(−) (12 μM; n = 2) suppressed the virus infection by a factor of approximately 20%. (B) The concentration dependency of InfA-3L/P95(−). InfA-3L/P95(−) reduced the infectivity at 4 and 8 μM. The neutralizing efficacy was approximately 30% at 4 μM. (n = 2). (C) The concentration dependency of InfA-6L/P95(−). InfA-6L/P95(−) infectivity was reduced in a concentration-dependent manner. The effective concentration was observed at 8 μM. The neutralizing efficacy was approximately 25%. (n = 4). (D) The concentration dependency of InfA-9L/P95(−). InfA-9L/P95(−) exhibited a neutralization effect in a concentration-dependent manner. The most effective concentration was 8 μM. The neutralizing efficacy was approximately 20%. (n = 2). (E) InfA-18L/wt and InfA-18L/P95(−). Both InfA-18L/wt (n = 4) and InfA-18L/P95(−) (n = 4) hardly showed the neutralization effect for the virus infection.
