Figure 2
From: TSWIFT, a novel method for iterative staining of embedded and mounted human brain sections
SHIELD protects tissue and allows for repeated staining of tissue with maintained signal. 20 × images from cycles of staining post-SHIELD. All are stained with DAPI at 50 µg/mL (Blue), Santa Cruz sc-58860 mouse mAb Tau (secondary 568) (Red) and DAKO Z0334 rabbit pAb GFAP (secondary 488) (Green). (A): cycle 1, (B): cycle 4, (C): cycle 7, (D): cycle 9.
