Figure 1 | Scientific Reports

Figure 1

From: AmiA and AliA peptide ligands, found in Klebsiella pneumoniae, are imported into pneumococci and alter the transcriptome

Figure 1

Growth suppression requires uptake of AmiA and AliA ligands via the Ami permease. The S. pneumoniae D39 parent strain was inhibited by AmiA ligand (AKTIKITQTR) and AliA ligand (FNEMQPIVDRQ), but inhibition was greatly reduced in the ∆amiC mutant. This indicates that the peptides are taken up via the Ami permease to act. Nonspecific ligand control, FNEMQPIVDRQAAKG, had little effect on growth of D39 parent strain or the ∆amiC mutant. Growth curves were performed in peptide-free chemically defined medium (CDM) by measuring optical density OD (450 nm) over time in absence or presence of peptide (0.5 mg/ml). Results represent 3 independent experiments, error bars indicate SEM.

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