Figure 7

ZnO NPs alter the expression level of genes involved in apoptosis and metastasis. (A) The relative fold change in expression levels of TP53, BAX, Caspase 3, and VIM of MDA-MB-231 cells after treatment with ½ IC₅₀ and IC₅₀ values of ZnO NPs for 24 h. RT-PCR analysis was presented as mean ± SD and analyzed using one-way ANOVA (turkey’s test); ****P-value signifies < 0.0001, ***P-value signifies < 0.001, **P-value signifies < 0.01, and *P-value signifies < 0.05. (B) Light microscopic images showed the expression levels of p53 and BCL-2 proteins in MDA-MB-231 cells via immunocytochemical staining after 24 h of treatment with½ IC₅₀ and IC₅₀ values of ZnO NPs.