Figure 7
From: Salvianolic acid A inhibits ferroptosis and protects against intracerebral hemorrhage
SAA treatment significantly reduced ferroptosis in primary cortical neurons through the Akt/GSK-3β/ Nrf2 pathway. (A) Western blot analysis was used to detect the expression of phosphorylated and total Akt. Phosphorylated and total GSK-3β, Nrf2 proteins, GPX4 proteins, and XCT proteins. (B) Semiquantitative measurements of the ratio of phosphorylated to total Akt. (C) Semiquantitative measurements of the ratio of phosphorylated to total GSK-3β. (D and E) The protein expression levels of nuclear Nrf2 and cytosolic Nrf2 were analyzed by Western blot. (F and H) Protein expression levels of GPX4 and XCT were analyzed by Western blot. Values are expressed as means ± SEM. ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05. n = 3/group.
