Figure 8
From: Salvianolic acid A inhibits ferroptosis and protects against intracerebral hemorrhage
The Akt pathway is necessary for SAA to induce GSK-3β phosphorylation and Nrf2 nuclear translocation in primary cortical neurons. These effects were inhibited by treatment with SH-6 (an AKT inhibitor) and ML385 (an Nrf2 inhibitor). (A) Western blot analysis was used to detect the expression of phosphorylated and total Akt. Phosphorylated and total GSK-3β, Nrf2 proteins, GPX4 proteins, and XCT proteins. (B–H) Western blot analysis was used to detect the expression of p-AKT, GSK-3β, Nrf2, GPX4, and XCT proteins after treatment with the inhibitor of the Akt/GSK-3β/ Nrf2 pathway. Values are expressed as means ± SEM. Data were analyzed using a one-way analysis of variance, followed by Tukey’s multiple comparison test. ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05. n = 3/group.
