Figure 1

TCF7L1 regulated genes are associated with cellular migration. (a) Diagram of TCF7L1 with protein interaction domains and engineered point mutations in the HMG DNA binding domain indicated. (b) Relative luciferase levels in HCT116, HT-29 and SW480 cells transfected with a TOPflash firefly luciferase reporter plasmid and plasmids encoding wild-type TCF7L1, TCF7L1^MUT, or vector as indicated. Luciferase values are normalized to Renilla luciferase levels. Data are represented as mean ± SEM (** p < 0.01, *** p < 0.001, ns, not significant). (c) Western blot analysis of exogenous TCF7L1 or TCF7L1^MUT in control (−Dox) or Dox-treated HCT116 cells. (d) Volcano plots of differentially expressed genes in TCF7L1-depleted and Dox-treated HCT116 cells expressing exogenous TCF7L1 or TCF7L1^MUT. TCF7L1-depleted HCT116 cells harboring a plasmid vector served as the control. Red points are genes whose expression is significantly downregulated and blue points are genes whose expression is significantly upregulated (p-adj < 0.05, |log2FoldChange|> 2). (e) Venn diagram comparing the number of downregulated or upregulated TCF7L1 or TCF7L1^MUT differentially expressed genes (DEGs). (f) Gene ontology (GO) analysis of the 397 genes whose expression was downregulated by TCF7L1. (g) Gene set enrichment analysis (GSEA) of TCF7L1 regulated genes within the hallmark epithelial-mesenchymal target gene set (Nominal p value = 0.0; normalized enrichment score =  − 1.78).