Figure 2
From: Increased water temperature contributes to a chondrogenesis response in the eyes of spotted wolffish
Histological features of spotted wolffish presenting with normal versus abnormal eyes. Whole eye sections were stained with Safranin O-fast green, a red/orange stain marking cartilage matrix. Areas of perichondrium (p) and maturing chondrocytes did not stain with safranin O-fast green. (A) Normal adult lumpfish whole eye shown for comparative purposes. (B) Normal spotted wolffish whole eye (the eye shown collapsed during paraffin embedding processing as with most spotted wolffish eyes). r, retina; sc, scleral cartilage. (C) Higher power view of the scleral cartilage of a spotted wolfish with a normal eye. Whole eye sections showing ocular deformation (G) and regression (H) associated with scleral cartilage lesion-like (scl) and retinal dysplasia (rd) from two separate spotted wolffish. (D), (E) and (F) Higher magnifications of scleral cartilage lesion-like tissue with isogenous groups of de novo cartilage (ig, arrowed). (I) Higher magnification of the eye in (H) showing globe-wide scleral cartilage lesion-like tissue traversing the center of the eye from posterior to anterior. Scale bars in (A), (B), (G), (H) are 5 milimeters. Whole eye sections in (A, B, G, H) are oriented with anterior facing left and posterior facing right. Panels (C–F) magnification: X200 ; panel I magnification: X5; scale bar subdivisions in (C) and (I) are 10 µm.
