Figure 7
From: Increased water temperature contributes to a chondrogenesis response in the eyes of spotted wolffish
Flow cytometric analysis of IgM and Sox9 in the rete mirabile and choroid body vascular organs of spotted wolffish. Flow cytometric analysis was performed on unstained cell preparations (A–D) and cell preparations stained with biotin-anti-IgM and rabbit monoclonal anti-Sox9 antibodies using streptavidin-phycoerythrine (PE) and anti-rabbit-alexafluor 647, respectively, as secondary conjugates (E–H) of the rete mirabile and choroid body vascular organs of spotted wolffish. Stained cells were analyzed for expression of IgM (F), Sox9 (G) and both IgM and Sox9 (H). (A), ungated cells. (F–H), stained cells gaited to exclude cellular debris and analyzed for IgM and Sox9 expression, as indicated. (B–D), unstained cells gated to exclude cellular debris and gated the same as the stained sets, as indicated. A representative experiment is shown.
