Figure 8
From: Increased water temperature contributes to a chondrogenesis response in the eyes of spotted wolffish
Histochemical morphological analysis of spotted wolffish scleral skeletal tissue after water temperature challenge. (A) Safranin O staining of scleral cartilage in spotted wolffish cultured in 1 °C, 8 °C and 14 °C water for 6 weeks as indicated. Two representative cases for 1 °C are shown to display the range of appearance of the tissue. Scale grid subdivisions indicate 10 µm for all panels. Images shown in (A) are representative of average values. Measurements of thickness of scleral cartilage including perichondrium (B) or not including perichondrium (C) for each temperature set (n = 4 animals per temperature set). Statistically significant differences (ANOVA, p ≤ 0.027) between groups was observed between 1 and 8 °C (ab), and between 1 and 14 °C (ac). No statistically significant differences in scleral cartilage diameter were observed between 8 and 14 °C.
