Figure 9
From: Increased water temperature contributes to a chondrogenesis response in the eyes of spotted wolffish
Immunohistochemical analysis of PCNA expression in spotted wolffish scleral cartilage tissue after water temperature challenge. (A) Representative PCNA staining of scleral cartilage in spotted wolffish cultured in 1 °C, 8 °C and 14 °C water for 6 weeks as indicated. Arrows in A, 8 °C panel, indicate PCNA positive mature chondrocytes. Bracket in A, 14 °C panel, indicates an area of chondrocytes expressing PCNA. Scale grid subdivisions indicate 10 µm for all panels in (A). (B) Quantitation of immunohistrochemistry analysis of PCNA positive cell number per scleral cartilage area (n = 4 animals per temperature set). Values are expressed as a percentage relatively to the average 8 °C value. Statistically significant difference (ANOVA, p ≤ 0.0044) was observed between 1 and 14 °C (ac), as well as between 8 and 14 °C (bc).
