Figure 1
From: Flavivirus Zika NS4A protein forms large oligomers in liposomes and in mild detergent
Crosslinking of ZIKV NS4A in lipid membranes. (A) Crosslinking of NS4A in PC/PS liposomes with DSS at the concentrations shown (lanes 1–7, see bottom of the gel) and purified non-crosslinked NS4A in DM detergent (lane 8); (B) crosslinking of NS4A in PC/PS liposomes with a fixed concentration (0.25 mM) of the crosslinkers shown (lanes 1–5) and purified non-crosslinked NS4A in DM detergent (lane 6). NS4A was detected by Western blot using anti-His-tag antibodies. The gel images are a composite of chemiluminescence and brightfield. Lane M is the MW marker, and lane M2 is lane M without chemiluminescence overlay, shown for clarity.
