Figure 6
From: Flavivirus Zika NS4A protein forms large oligomers in liposomes and in mild detergent
Alphafold-2 structure prediction of dimeric NS4A. (A) Side view of predicted rank 1 model of NS4A homodimer colored according to plDDT scores (see color bar). Confidence was relatively high (pLDDT ~ 80) for residues ~ 20–60 (mostly α3 and α4), but low (pLDDT ~ 50) for the rest of the molecule; (B) top view of (A); (C) PAE plot; (D) predicted stabilizing salt bridges between E50 and E53 in one monomer (magenta) and R20 in the other (cyan). Distances are shown in Å; (E,F) representation of the N-terminal 62 residues involved in the homomeric interaction shown in side-view (E) and top-view (F). One monomer is shown as hydrophobicity surface (80% transparency) and the other in liquorice representation (preset in Chimera X). The residues facing the other monomer are indicated.
