Figure 6
NLRC5 induces dMMR status by suppress NF-κB pathway in EC. (A,B) The protein expression levels of p65 and p-p65 in HEC-1B and Ishikawa cell lines were examined by western blot. The protein expression levels were quantified using the ImageJ software and normalized using p65 protein levels (mean ± SD, n = 3). (C) The mRNA expression levels of MMR genes in HEC-1B and Ishikawa cell lines were examined by qRT-PCR (mean ± SD, n = 3). (D) The protein expression levels of MMR genes in HEC-1B and Ishikawa cell lines were examined by western blot. The protein expression levels were quantified using the ImageJ software and normalized using β-actin protein levels (mean ± SD, n = 3). The corresponding brightfield images are included in the supplementary material. Data represent mean ± SD, *P < 0.05 versus control, **P < 0.01 versus control, ***P < 0.001 versus control, ****P < 0.0001 versus control. #P < 0.05 versus NLRC5, ##P < 0.01 versus NLRC5, ###P < 0.001 versus NLRC5, ####P < 0.0001 versus NLRC5.
