Table 1 Features of the MT-50.1 and MT-50.4 cell lines.

From: Novel paired CD13-negative (MT-50.1) and CD13-positive (MT-50.4) HTLV-1-infected T-cell lines with differential regulatory T cell-like activity

Parameter

Features

MT-50.1

MT-50.4

Clinical data

 Patient

68-year-old woman

 Diagnosis

Lymphoma-type ATL

 Sample

Peripheral blood

 Sample collection time

At diagnosis

 Year of establishment

2018

Cell line

 Authentication

Yes (by STR DNA fingerprinting)

 Culture medium

RPMI 1640 supplemented with FBS (10%) and human IL-2 (100 U/mL)

 Growth pattern

Single-cell suspension with cell aggregates

 Cryopreservation

CELLBANKER (Takara Bio)

 Morphology

Medium-to-large-sized cells with round or slightly irregular nuclei

 Viral status

Polyclonal integration of the HTLV-1 genome

Expression of HTLV-1 tax and HBZ

+

Doubling time

18 h

24 h

Karyotype

46, XX, der(13)t(2;13)(q11.2;q34)

46, XX

Immunoprofile

CD4+CD13−CD25+

CD4+CD13+CD25+

Secretion of IL-10

−

+

Secretion of TGF-β

Below the detection limita

+

Suppression effect on CD4+ T cells

Lowerb

Higherb

Efficacy of bestatin on cell growth inhibition

−

+

  1. ATL, adult T-cell leukemia/lymphoma; FBS, fetal bovine serum; HTLV-1, human T-cell leukemia virus type-1; IL-2, interleukin-2; STR, short tandem repeat; TGF-β, transforming growth factor-β.
  2. aELISA assay using human TGF-β DuoSet ELISA kit.
  3. bComparison between the MT-50.1 and MT-50.4 cells.
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