Figure 3 | Scientific Reports

Figure 3

From: The matricellular protein CCN5 prevents anti-VEGF drug-induced epithelial-mesenchymal transition of retinal pigment epithelium

Figure 3The alternative text for this image may have been generated using AI.

Recombinant CCN5 protein inhibits EMT induced by aflibercept (Afl) in mouse eyes. (A) An experimental scheme. Normal human IgG (40 mg/eye), Afl (40 μg/eye), and Afl and CCN5 (40 ng/eye) were intravitreally injected. Mice were sacrificed, and eyes were enucleated at 14 days post-injection. (B) Flat mounts of RPE/choroid complex were stained with anti-α-SMA antibody. Representative images are shown. Scale bar, 1000, 500, and 250 μm. (C) The intensity of α-SMA positive areas was quantified by Image-J software and plotted. (D) Flat mounts of RPE/choroid complex were stained with anti-α-SMA and anti-ZO-1 antibodies, and Hoechst 33342. Representative images are shown. Scale bar, 50 μm. (E) The mean number of RPE cells in 100 × 100 μm area was manually counted and plotted. (F) The mean size of RPE cells in 100 × 100 μm area was manually calculated and plotted. Error bars = SEM. (n = 8, Cont; n = 8, IgG; n = 10, Afl; n = 13, Afl+CCN5; one-way ANOVA; ***p < 0.001).

Back to article page