Figure 1
From: IRAK4 is an immunological checkpoint in neuropsychiatric systemic lupus erythematosus
IRAK4 mutation abrogates NPSLE symptoms in MRL mice. (A) Splenocytes (top panels) and spleen weight (bottom panels) of 6 month-old female (left panels) and male (right panels) MRL, MRL-IRAK4-KD, C57BL/6, and IRAK4-KD mice were reported. Each dot represents one mouse. Sera were obtained from female MRL, MRL-IRAK4-KD, C57BL/6, and IRAK4-KD mice and quantified for (B) anti-dsDNA IgG antibodies and (C) CCL5 levels. Data are shown as mean +/− SEM. Each dot represents one mouse. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (one-way ANOVA). (D) Morris water maze (MWM) tests were applied to 10 month-old mice. Specifically, mice were tested for 3 consecutive days, rested for 2 days and tested daily for 4 additional days. n = 10–13/group. A mixed-effects model was used to compare differences in time-to-platform between the two groups after accounting for the repeated measures correlation52. We calculated overall mean difference between MRL and MRL-IRAK4-KD mice (two left panels) and 95% confidence interval (CI) during the observation period: days 1–3: on average, the difference was 6.05 s (95% CI 1.62–10.48) (p = 0.010). Days 6–9: on average, the difference was 7.26 s (95% CI 3.54–10.98) (p < 0.001). MWM analysis of all the mice strains is shown on the two right panels.
