Figure 5

Macrophages containing replicating S. Typhi had elevated phosphorylation of STAT3 at the serine 727 residue. THP-1 macrophages were infected with S. Typhi pFCcGi. At 4- and 18-h post-infection (hpi), cells were collected and STAT3 phosphorylation at Ser727 was measured via phospho-flow cytometry⁵⁴. The histogram depicts fluorescence of pSTAT3^Ser727 in macrophages containing non-replicating S. Typhi (yellow), macrophages containing replicating S. Typhi (blue), and macrophages serving as an unstained control (dotted line). Median fluorescence intensity of pSTAT3^Ser727 in the two populations of challenged macrophages at 4- and 18-hpi is shown in the bar plots to the right of the histogram. Data shown includes samples from independent experiments; n = 2 for both experiments.