Figure 4
From: Investigating the potential of X chromosome shredding for mouse genetic biocontrol
Transgenic mice co-expressing X-shredder gRNAs and germline promoter-Cas9 did not generate altered male bias. (a) Schematic showing the method to determine if male bias was occurring in offspring of germline promoter-Cas9Tg; X-shredderTg male × WT females (created with Biorender.com). Control crosses consisted of germline promoter-Cas9Tg; X-shredderTg females × WT males. Enumeration of female and male pups from: (b) Ccna1Tg; X-BTg × WT matings. Control matings = 11 plugs from three transgenic females with 10 litters, experimental matings = 29 plugs from three transgenic males with 22 litters. (c) Ccna1Tg; X-CTg × WT matings (two independent X-CTg founder lines). Ccna1Tg; X-C-1Tg control matings = 4 plugs from one transgenic female with 3 litters, Ccna1Tg; X-C-1Tg experimental matings = 28 plugs from one transgenic male with 15 litters. Ccna1Tg; X-C-2Tg control matings = 3 plugs from one transgenic female with 3 litters, Ccna1Tg; X-C-2Tg experimental matings = 29 plugs from two transgenic males with 0 litters. (d) Ccna1Tg; X-DTg × WT matings. Control matings = 7 plugs from three transgenic females with 8 litters, experimental matings = 109 plugs from three transgenic males with 48 litters. (e) Prm1Tg; X-CTg × WT matings (two independent X-CTg founder lines). Prm1Tg; X-C-1Tg experimental matings = 16 plugs from one transgenic male with 14 litters. Prm1Tg; X-C-2Tg experimental matings = 42 plugs from three transgenic males with 27 litters. (f) Stra8Tg; X-CTg × WT matings (two independent X-CTg founder lines). Stra8Tg; X-C-1Tg experimental matings = 16 plugs from one transgenic male with 10 litters. Stra8Tg; X-C-2Tg experimental matings = 20 plugs from three transgenic males with 18 litters. (b–f) Numbers in bars indicate number of female pups (white) and male pups (blue) combined from litters of the same genotype. Chi squared test.
