Figure 5
From: Investigating the potential of X chromosome shredding for mouse genetic biocontrol
Functional assessment of Ccna1-Cas9Tg; X-shredderTg triple transgenic mice. Pregnancy success as determined by plug and successful pregnancy counts (left) and enumeration of male and female pups (right) from: (a) Ccna1Tg; X-BTg; X-CTg × WT matings. Control matings = 2 plugs from one transgenic female with 2 litters, experimental matings = 25 plugs from three transgenic males with zero litters. (b) Ccna1Tg; X-BTg; X-DTg × WT matings. Control matings = 1 plug from one transgenic female with 1 litter, experimental matings = 10 plugs from one transgenic male with zero litters. (c) Ccna1Tg; X-CTg; X-DTg × WT matings. Control matings = 3 plugs from one transgenic female with 3 litters, experimental matings = 44 plugs from five transgenic males with zero litters. (d) H&E staining of representative testis sections from mice used in (a), (b) and (c). Mice 15–17 weeks of age. Scale bar = 100 µM.
