Table 2 Primers and probes used for Coronaviridae, bovine coronavirus (BCoV) and mammal β Actin gene fragment as internal control amplification detection.
From: Prevalence of coronaviruses in European bison (Bison bonasus) in Poland
Target | Primer/probe | Sequence (5′–3′) | Amplicon size (bp) | Gene/protein | Reference |
|---|---|---|---|---|---|
Pancoronavirus | Chu-RdRp-N1-F | GGKTGGGAYTAYCCKAARTG | 602 | RdRp | |
Chu-RdRp-N1-R | TGYTGTSWRCARAAYTCRTG | ||||
Chu-RdRp-N2-F | GGTTGGGACTATCCTAAGTGTGA | 440 | |||
Chu-RdRp-N2-R | CCATCATCAGATAGAATCATCAT | ||||
BCoV | BCoV-F | CTGGAAGTTGGTGGAGTT | 85 | M/matrix | |
BCoV-R | ATTATCGGCCTAACATACATC | ||||
BCoV-Pb | FAM-CCTTCATATCTATACACATCAAGTTGTT-BHQ1 | ||||
Sp1 | CTTATAAGTGCCCCCAAACTAAAT | 622 | S/spike | ||
Sp2 | CCTACTGTGAGATCACATGTTTG | ||||
β Actin | ACT-1005-F | CAGCACAATGAAGATCAAGATCATC | 130 | bACT | |
ACT-1135-R | CGGACTCATCGTACTCCTGCTT | ||||
ACT-1081-HEX | HEX-TCGCTGTCCACCTTCCAGCAGATGT- BHQ1 |
